A walk-through of the equipment, methods, and quality thresholds that every Freedom Aminos compound passes before it ships. No rounding, no shortcuts, no "close enough."
Each method confirms a different property — purity, identity, and stability. A batch that fails any one is rejected.
A reverse-phase column separates the compound from impurities. A UV detector reads the eluent at the absorption wavelength of peptide bonds (typically 214 nm). Each peak is integrated, area is normalized, and purity is reported as the percentage of total chromatographic area.
The compound is ionized via electrospray (ESI) and the resulting ions are mass-separated by a quadrupole-orbitrap. Observed mass is compared to theoretical exact mass — a match within 5 ppm confirms molecular identity. Fragmentation patterns rule out close-mass impurities.
Each batch is freeze-dried under controlled vacuum to a residual moisture target below 2%. Karl Fischer titration confirms moisture content. Vials are sealed under nitrogen, then a sample subset is held at -20°C, 4°C, and 25°C for periodic re-test against the original COA.
Calibrated, certified, and maintained on documented schedules. Every result is traceable to instrument, operator, and date.
Quaternary pump, autosampler, diode-array UV/Vis detector. Calibrated quarterly against NIST-traceable standards.
Quadrupole-orbitrap mass spectrometer with HCD fragmentation. Mass accuracy verified daily with calibration mix.
Volumetric Karl Fischer titrator for residual moisture analysis. Resolution to 1 ppm; certified annually.
Lyophilizer with -85°C condenser and shelf temperature control. Residual moisture profiles logged for every cycle.
| Parameter | Specification |
|---|---|
| Purity floor (HPLC area %) | 99.0% |
| Mass accuracy (ppm tolerance) | < 5 |
| Residual moisture (Karl Fischer) | < 2.0% |
| Bioburden (CFU/mg, trypticase soy) | < 10 |
| Endotoxin (EU/mg, LAL) | < 0.5 |
| Storage temperature, long-term | -4°F |
| Stability monitoring interval | 3 / 6 / 12 mo |
| Per-batch retain sample, sealed | Yes |
A purity number on a label means nothing without the chromatogram behind it. We publish both — every time, for every batch — because researchers should never have to take our word for it.
Every batch publishes a Certificate of Analysis. Browse the catalog, pick a compound, and you can verify the science behind it before you order.